High pressure freezer
HPM Live μ developed and manufactured by CryoCapCell is an advanced, automated high pressure freezing system for the vitrification of specimens. HPM Live μ can be used for the vitrification of a wide range of cell and tissue types – a range of holders enables selection of the optimal holder for each specimen type. HPM Live μ is the only high pressure freezer to enable live correlative light and electron microscopy (CLEM) experiments.
- Automatic specimen transfer in 1.26 seconds
- Start-up cycle fully controlled by HPM Live μ
- Preparation cycle lasts 20 minutes
- User defined parameters
- 15” inch HD colour touchscreen
- High pressure freezing data and curves are automatically saved and can be sent by e-mail
- Vitrification of two successive samples in 45 seconds
- Optimized workflow using CryoCapsule®
- Open system and flexible to use – also compatible with a range of sample carriers
- Remote control and online diagnostics if connected to internet
Rapid transfer in 1.26 seconds
CLEM by high pressure freezing enables researchers to go beyond human speed and relate a live cellular event to its ultrastructure. HPM Live μ is a uniquely optimises the entire workflow of CLEM utilising vitrification by high pressure freezing. At the heart of the process is the CryoCapsule which comprises a special sapphire disc printed with carbon coordinates and a gold spacer maintained together by a polyethylene ring. The CryoCapsule is entirely harmless to biological material and protects the specimen against mechanical damage during specimen preparation.
HPM Live µ is the first high pressure freezing system that can be interfaced directly to a confocal / fluorescence / light microscope to allow the specimen to be examined live before initiating the vitrification process.
HPM Live µ integrates an automated specimen arm with a light microscope by holding the CryoCapsule containing the specimen above the objective. At the appropriate time the arm is activated and in less than two seconds the specimen is vitrified.
Specimen holders and clamps
HPM Live u offers a choice of specimen holders to suit most applications. In addition to CryoCapsule the HPM Live u high pressure freezer can be used with standard specimen discs (3mm and 6mm), sapphire discs and copper tubes.
Each specimen holder uses a dedicated clamp to ensure optimal positioning for the vitrification process.
Applications examples (PDFs)
To configure a working HPM Live u you will require the following:
- HPM Live u main instrument
- Specimen clamps of your choice
- Specimen holders to fit clamps
- Liquid Nitrogen dewar (if required)
- Additional accessories
Please add your required items using the quote buttons below and we will endeavour to provide your quotation as quickly as possible
Specification of High Pressure Freezer Live μ
- Width: 995,5mm
- Depth: 1064mm
- Height: 1855mm
- Weight: 450kg
- Voltage: 240V
- Installed power: 7000W
- Dry air gas at 6 bars/87psi
- LN2 dewar capacity: 9 litres
Instrument connected to a nitrogen container of 60 to 100 litres
- LN2 consumption: 25 litres per day, including cooling
- Alcohol reservoir: 0.5 litre
Ordering information:
Main instruments
To configure a working HPM Live u or HMP Alpha system please select the main instrument and specimen holders to your quote and then add any other accessories from the list of options below:
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HPM Live – Automated High Pressure Freezer
HPM Live µ automated high pressure machine for vitrification in 1.26 seconds, with remote control and online diagnostics (if connected to internet).
- Automatic transfer in 1-26 seconds
- The only existing commercial solution for live CLEM (Correlative Light and Electron Microscopy)
- Start-up cycle fully controlled by HPM Live
- 20-minute preparation cycle
- Live samples imaging up to 10 minutes
- Vitrification of two successive samples in 45 seconds
- Data and curves are automatically saved and sent by e-mail
- Optimised for CryoCapsules®
- Open system – flexible to use and compatible with various sample carriers (carriers of 3, 4 or 6mm tubes etc. existing in the market)
- Each user has his own pre-set parameters
- 15” inch HD colour touchscreen
- 1 x clamp 3mm diameter, 1 x clamp for CryoCapsules®
- Station for unloading samples
- 1 kit of spare parts (3 x heated collars, thermocouple sensor, 10 seals)
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HPM Alpha – Manual High Pressure Freezer
The HPM Alpha is a manual high pressure freezer for the vitrification of
biological samples.
- Start-up cycle fully controlled by HPM ⍺
- 20-minute preparation cycle
- Vitrification of two successive samples in 45 seconds
- Each user can set their own pre-set parameters
- 15” inch HD colour touchscreen
- Data and curves are automatically saved
- Open system which is flexible to use: also compatible with various sample carriers (3mm, 4,5mm or 6mm, tubes, etc.) existing in the market
- 1 clamp 3mm diameter
- Unloading sample station
Specimen clamps
Please select specimen clamps from the options below and then select the corresponding specimen holders:
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Clamp CC
Specially designed for the exclusive CryoCapsule.
Price On Request £0.00 Add to basket -
Clamp C3
Specially designed for the Ø3mm carriers.
Price On Request £0.00 Add to basket -
Clamp C6
Specially designed for the Ø6mm carriers.
Price On Request £0.00 Add to basket -
Clamp CT
Specially designed for the tubes.
Price On Request £0.00 Add to basket
Specimen carriers
Please select the appropriate carriers for your clamps selected from above:
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CryoCapsule ©
This exclusive tool is specially developed for Live imaging before freezing.
Price On Request £0.00 Add to basket -
Carrier 3A
Ø 3 Specimen Carrier (Type A) Cavity 0.1 / 0.2 mm (x50)
Price On Request £0.00 Add to basket -
Carrier 3B
Ø 3 Specimen Carrier (Type B) Cavity 0.3 mm (x50)
Price On Request £0.00 Add to basket -
Carrier 6A
Ø 6 Specimen Carrier (Type A) Cavity 0.1 / 0.2 mm (x50)
Price On Request £0.00 Add to basket -
Carrier 6B
Ø 6 Specimen Carrier (Type B) Cavity 0.3 mm (x50)
Price On Request £0.00 Add to basket -
Copper tube 0.6
Copper tube Ø 0.6 / 0.3 mm x 9 mm (x20)
Price On Request £0.00 Add to basket -
Copper tube 0.5
Copper tube Ø 0.5 / 0.35 mm x 9 mm (x20)
Price On Request £0.00 Add to basket -
Sapphire disc 3
Sapphire disc Ø 3 x0.05mm (x20)
Price On Request £0.00 Add to basket -
Sapphire disc 6
Sapphire disc Ø 6 x0.10mm (x20)
Price On Request £0.00 Add to basket
HPM liquid nitrogen dewars
A liquid nitrogen supply dewar is required to run the HPM Live u. If you do not have an existing dewar please select on of the options below:
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Liquid Nitrogen dewar 60l
Useful capacity (l): 58 -Total capacity (l): 60- Total Height (mm): 915- Inner height (mm): 698- Outer diameter (mm)460- Empty weight (kg): 38- Full weight (kg): 86.5- Static consumption (%/day): 1.8- Max. working pressure (bar) : 1.5- Roller base- insulated flexible hose
Price On Request £0.00 Add to basket -
Liquid Nitrogen dewar 120l
Useful capacity (l): 120- Total capacity (l): 127- Total Height (mm): 1045- Inner height (mm): 853- Outer diameter (mm)570- Empty weight (kg): 62- Full weight (kg): 159- Static consumption (%/day): 1.3- Max. working pressure (bar) : 1.5- Roller base- insulated flexible hose
Price On Request £0.00 Add to basket -
Liquid Nitrogen level indicator
When an accurate indication of the remaining liquid nitrogen is needed- a digital level indicator can be mounted instead of the float level gauge.
Price On Request £0.00 Add to basket
HPM accessories
For optimal use of the HPM Live u it is sometimes useful to have additional accessories from the options below:
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Loading station
The loading station is a convenient tool to load your sample contained in Cryocapsules- classical carriers from Ø3mm to Ø6mm or tubes. It is furnished without the clamp holder.
Price On Request £0.00 Add to basket -
Unloading station
The unloading station allows you to easily unscrew the clamp attached to the clamp holder.
Price On Request £0.00 Add to basket -
Storage station
The storage station keeps your samples well organized in the liquid nitrogen. It is compatible with cryotubes.
Price On Request £0.00 Add to basket -
Stereomicroscope
Stereomicroscope Zeiss Stemi 305 EDU, Greenough system with stereoscopic angle of 11 °, zZoom 5:1 (0-8x…4-0x), zoom with clickstops on positions 0-8x-1x-2x-3x4x, free working distance 110 mm, angle of vision with adjustable interocular distance 55…75 mm
Price On Request £0.00 Add to basket -
Cleaning accessory
Swab for the vitrification chamber cleaning.
Price On Request £0.00 Add to basket -
R221 high contrast embedding resin for CLEM
R221 is a high contrast embedding resin for volume CLEM (100ml bottle). The new R221 resin preserves fluorescence- evacuate charging effects at the surface with only traces of UA. Developed for TEM and SEM applications.
£251.91 Add to basket -
Small dewar for liquid nitrogen
The clamp holder is collected into this dewar following the vitrification process. Supplementary dewars allow to speed up the vitrification session.
Price On Request £0.00 Add to basket